1ZX4
Structure of ParB bound to DNA
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 8.2.2 |
| Synchrotron site | ALS |
| Beamline | 8.2.2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-03-23 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 1.006 |
| Spacegroup name | P 62 2 2 |
| Unit cell lengths | 154.600, 154.600, 132.300 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 50.610 - 2.980 |
| R-factor | 0.2481 |
| Rwork | 0.248 |
| R-free | 0.29600 |
| Structure solution method | MAD |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.600 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | SOLVE |
| Refinement software | CNS (1.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 65.900 | 3.150 |
| High resolution limit [Å] | 2.980 | 2.980 |
| Rmerge | 0.092 | 0.430 |
| Number of reflections | 18057 | |
| <I/σ(I)> | 7 | 1.8 |
| Completeness [%] | 92.0 | 92 |
| Redundancy | 3 | 2.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 298 | sodium citrate, imidazole, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | sodium citrate | ||
| 2 | 1 | 2 | imidazole | ||
| 3 | 1 | 2 | sodium citrate |
