1ZX2

Crystal Structure of Yeast UBP3-associated Protein BRE5

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X25
Synchrotron site	NSLS
Beamline	X25
Temperature [K]	100
Detector technology	CCD
Collection date	2004-10-12
Detector	ADSC QUANTUM 4
Wavelength(s)	0.9795, 0.9794, 0.9500, 0.9790
Spacegroup name	P 61 2 2
Unit cell lengths	90.909, 90.909, 194.896
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	79.060 - 2.100
R-factor	0.1896
Rwork	0.188
R-free	0.21299
Structure solution method	MAD
RMSD bond length	0.032
RMSD bond angle	2.396
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	SOLVE
Refinement software	REFMAC (5.1.24)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.180
High resolution limit [Å]	2.100	2.100
Rmerge	0.076	0.379
Number of reflections	28566
<I/σ(I)>	28.3	8.7
Completeness [%]	99.2	99.6
Redundancy	10.4	10.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5.5	300	PEG 3350, Ammonium Sulfate, pH 5.5, VAPOR DIFFUSION, HANGING DROP, temperature 300K