1ZWS
Crystal structure of the catalytic domain of human DRP-1 kinase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | MAX II BEAMLINE I711 |
| Synchrotron site | MAX II |
| Beamline | I711 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2004-09-27 |
| Detector | MARRESEARCH |
| Wavelength(s) | 1.095 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 52.620, 211.830, 128.420 |
| Unit cell angles | 90.00, 101.82, 90.00 |
Refinement procedure
| Resolution | 20.000 - 2.900 |
| Rwork | 0.220 |
| R-free | 0.25760 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1sxo |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.300 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | MOLREP |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 3.000 |
| High resolution limit [Å] | 2.900 | 2.900 |
| Number of reflections | 55013 | |
| <I/σ(I)> | 6.1 | 1.5 |
| Completeness [%] | 90.5 | 84.2 |
| Redundancy | 1.9 | 1.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 295 | Tris, PEG, magnesium chloride, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 295K |
