1ZWJ

X-ray structure of galt-like protein from arabidopsis thaliana AT5G18200

Replaces:  1VKV

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 14-ID-B
Synchrotron site	APS
Beamline	14-ID-B
Temperature [K]	100
Detector technology	CCD
Collection date	2004-02-11
Detector	MAR CCD 165 mm
Wavelength(s)	0.9786
Spacegroup name	P 21 21 21
Unit cell lengths	59.984, 95.535, 110.522
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	27.590 - 2.300
Rwork	0.215
R-free	0.26900
Structure solution method	MOLECULAR REPLACEMENT, SAD
Starting model (for MR)	1gup
RMSD bond length	0.006
RMSD bond angle	1.300
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	CNS
Refinement software	CNS (1.1)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	2.350
High resolution limit [Å]	2.300	5.670	2.300
Rmerge	0.044	0.023	0.406
Number of reflections	27575
<I/σ(I)>	24.03		3.646
Completeness [%]	95.6		80.4
Redundancy	6.4	6.8	5.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5.5	293	10 MG/ML PROTEIN, 20% PEG 2K, 0.2M SODIUM CHLORIDE, 0.1M MES-ACETATE, pH 5.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K
1	VAPOR DIFFUSION, HANGING DROP	5.5	293	10 MG/ML PROTEIN, 20% PEG 2K, 0.2M SODIUM CHLORIDE, 0.1M MES-ACETATE, pH 5.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K
1	VAPOR DIFFUSION, HANGING DROP	5.5	293	10 MG/ML PROTEIN, 20% PEG 2K, 0.2M SODIUM CHLORIDE, 0.1M MES-ACETATE, pH 5.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K