1ZWI

Structure of mutant KcsA potassium channel

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X29A
Synchrotron site	NSLS
Beamline	X29A
Temperature [K]	150
Detector technology	CCD
Collection date	2005-04-09
Detector	ADSC QUANTUM 4
Wavelength(s)	0.98
Spacegroup name	I 4
Unit cell lengths	155.980, 155.980, 75.960
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	50.000 - 2.500
R-factor	0.256
Rwork	0.243
R-free	0.25600
Structure solution method	MOLECULAR REPLACEMENT
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	CNS
Refinement software	CNS

Data quality characteristics

	Overall
Low resolution limit [Å]	50.000
High resolution limit [Å]	2.500
Number of reflections	27186
Completeness [%]	85.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1
1