1ZW6
Crystal Structure of the GTP-bound form of RasQ61G
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X26C |
| Synchrotron site | NSLS |
| Beamline | X26C |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2004-12-06 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 1.0 |
| Spacegroup name | H 3 2 |
| Unit cell lengths | 88.776, 88.776, 134.392 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 30.790 - 1.500 |
| R-factor | 0.146 |
| Rwork | 0.144 |
| R-free | 0.17403 |
| Structure solution method | SAD |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.489 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | SHELX |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.800 | 1.540 |
| High resolution limit [Å] | 1.500 | 1.500 |
| Number of reflections | 30980 | |
| <I/σ(I)> | 24.1 | 3.8 |
| Completeness [%] | 99.4 | 99.8 |
| Redundancy | 7.1 | 6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 293 | 22% PEG4000, 200mM Ca Acetate, 100 mM Tris-HCl, 10 mM MgCl2, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
