1ZVZ
Vinculin Head (0-258) in Complex with the Talin Rod Residue 820-844
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-2 |
| Synchrotron site | ESRF |
| Beamline | ID14-2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-03-13 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.934 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 36.013, 52.164, 153.841 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 26.290 - 1.800 |
| R-factor | 0.24095 |
| Rwork | 0.239 |
| R-free | 0.27636 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1xwj |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.097 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 1.860 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rmerge | 0.078 | 0.346 |
| <I/σ(I)> | 16.1 | 2.09 |
| Completeness [%] | 97.7 | 95.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | VAPOR DIFFUSION, SITTING DROP, temperature 293K |
