1ZVN

Crystal structure of chick MN-cadherin EC1

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X4A
Synchrotron site	NSLS
Beamline	X4A
Temperature [K]	110
Detector technology	CCD
Collection date	2002-06-09
Detector	ADSC QUANTUM 4
Wavelength(s)	1.065
Spacegroup name	P 1 21 1
Unit cell lengths	32.322, 76.155, 37.073
Unit cell angles	90.00, 96.01, 90.00

Refinement procedure

Resolution	19.730 - 2.160
R-factor	0.17264
Rwork	0.169
R-free	0.24240
Structure solution method	SAD
RMSD bond length	0.016
RMSD bond angle	2.253
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	SOLVE
Refinement software	REFMAC (5.2.0005)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000	2.240
High resolution limit [Å]	2.160	2.160
Rmerge	0.076	0.252
Number of reflections	9573
<I/σ(I)>	9.77
Completeness [%]	100.0	93

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6	277	60% saturated ammonium sulfate, 0.1M Bis-Tris pH 6.0, 7mM YbCl3 , VAPOR DIFFUSION, HANGING DROP, temperature 277K
1	VAPOR DIFFUSION, HANGING DROP	6	277	60% saturated ammonium sulfate, 0.1M Bis-Tris pH 6.0, 7mM YbCl3 , VAPOR DIFFUSION, HANGING DROP, temperature 277K
1	VAPOR DIFFUSION, HANGING DROP	6	277	60% saturated ammonium sulfate, 0.1M Bis-Tris pH 6.0, 7mM YbCl3 , VAPOR DIFFUSION, HANGING DROP, temperature 277K