1ZVJ
Structure of Kumamolisin-AS mutant, D164N
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Detector | MARRESEARCH |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 1 |
| Unit cell lengths | 41.850, 44.660, 49.070 |
| Unit cell angles | 114.90, 105.90, 102.10 |
Refinement procedure
| Resolution | 10.000 - 2.030 |
| R-factor | 0.1881 |
| Rwork | 0.184 |
| R-free | 0.26300 |
| Structure solution method | AB INITIO |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.019 |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | SHELXL-97 |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.090 |
| High resolution limit [Å] | 2.020 | 2.020 |
| Number of reflections | 17485 | |
| <I/σ(I)> | 20.3 | 5.5 |
| Completeness [%] | 92.7 | 76.1 |
| Redundancy | 2.1 | 1.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.2 | 298 | PEG8000, Ammounium Sulfate, pH 4.2, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
