1ZVH
Crystal structure of the VHH domain D2-L24 in complex with hen egg white lysozyme
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | EMBL/DESY, HAMBURG BEAMLINE BW7A |
| Synchrotron site | EMBL/DESY, HAMBURG |
| Beamline | BW7A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2002-02-10 |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.97600 |
| Spacegroup name | I 4 |
| Unit cell lengths | 114.782, 114.782, 33.273 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 14.710 - 1.500 |
| R-factor | 0.208 |
| Rwork | 0.208 |
| R-free | 0.23400 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | pdb entries 1MEL and 1BZQ |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.600 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 15.000 | 1.550 |
| High resolution limit [Å] | 1.500 | 1.500 |
| Rmerge | 0.061 | 0.353 |
| Number of reflections | 35116 | |
| Completeness [%] | 99.9 | 99.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.4 | 293 | Ammonium Sulphate, PEG 8000, glycerol, pH 7.4, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
