1ZV7
A structure-based mechanism of SARS virus membrane fusion
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X4A |
| Synchrotron site | NSLS |
| Beamline | X4A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2003-09-12 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 1.0055,,0.9200, 0.9203, 0.9102 |
| Spacegroup name | C 2 2 2 |
| Unit cell lengths | 43.650, 90.213, 42.256 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 45.200 - 1.700 |
| R-factor | 0.211 |
| Rwork | 0.209 |
| R-free | 0.25800 |
| Structure solution method | MAD |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.564 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SOLVE |
| Refinement software | REFMAC (5.1.24) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 45.200 | 1.760 |
| High resolution limit [Å] | 1.700 | 1.700 |
| Rmerge | 0.063 | 0.382 |
| Number of reflections | 9539 | |
| <I/σ(I)> | 16.5 | 5.7 |
| Completeness [%] | 99.9 | 99.9 |
| Redundancy | 7 | 7.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 298 | PEG MME 2000, Nickel chloride, Tris-HCL, pH 8.50, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
