1ZV1

Crystal structure of the dimerization domain of doublesex protein from D. melanogaster

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X9B
Synchrotron site	NSLS
Beamline	X9B
Temperature [K]	200
Detector technology	CCD
Collection date	2003-04-11
Detector	ADSC QUANTUM 4
Wavelength(s)	0.9788
Spacegroup name	P 21 21 21
Unit cell lengths	39.773, 46.623, 59.771
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	10.860 - 1.600
R-factor	0.21084
Rwork	0.208
R-free	0.25722
Structure solution method	SAD
RMSD bond length	0.013
RMSD bond angle	1.394
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	SHELXS
Refinement software	REFMAC (5.2.0005)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000	1.660
High resolution limit [Å]	1.600	1.600
Rmerge	0.038	0.182
Number of reflections	15159
<I/σ(I)>	62.3	11
Completeness [%]		96.9
Redundancy	10.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.4	298	ammonium sulfate, isopropanol, tris, sodium chloride , pH 7.4, VAPOR DIFFUSION, HANGING DROP, temperature 298K
1	VAPOR DIFFUSION, HANGING DROP	7.4	298	ammonium sulfate, isopropanol, tris, sodium chloride , pH 7.4, VAPOR DIFFUSION, HANGING DROP, temperature 298K