1ZUY
High-resolution structure of yeast Myo5 SH3 domain
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | EMBL/DESY, HAMBURG BEAMLINE X11 |
| Synchrotron site | EMBL/DESY, HAMBURG |
| Beamline | X11 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2004-02-03 |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.813 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 27.060, 42.250, 90.910 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 20.000 - 1.390 |
| R-factor | 0.16829 |
| Rwork | 0.166 |
| R-free | 0.20581 |
| Structure solution method | MAD |
| RMSD bond length | 0.017 |
| RMSD bond angle | 1.752 |
| Data scaling software | XDS |
| Phasing software | SOLVE |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 1.430 |
| High resolution limit [Å] | 1.390 | 1.390 |
| Number of reflections | 21535 | |
| <I/σ(I)> | 20 | 3.3 |
| Completeness [%] | 99.0 | 90.6 |
| Redundancy | 6.1 | 3.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 295 | ammonium sulfate, isopropanol, VAPOR DIFFUSION, HANGING DROP, temperature 295K |
