1ZUW
Crystal structure of B.subtilis glutamate racemase (RacE) with D-Glu
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | SRS BEAMLINE PX14.1 |
| Synchrotron site | SRS |
| Beamline | PX14.1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 1.488 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 134.120, 60.274, 125.271 |
| Unit cell angles | 90.00, 117.34, 90.00 |
Refinement procedure
| Resolution | 10.000 - 1.750 |
| Rwork | 0.179 |
| R-free | 0.23720 |
| Structure solution method | MAD |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.620 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SHELXS |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 10.000 | 1.790 |
| High resolution limit [Å] | 1.750 | 1.750 |
| Rmerge | 0.053 | 0.390 |
| Number of reflections | 82597 | |
| <I/σ(I)> | 14 | 1.9 |
| Completeness [%] | 97.0 | 98 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 290 | 16% PEG 3350, 0.2 M di-Ammonium tartrate , pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 290K |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 290 | 16% PEG 3350, 0.2 M di-Ammonium tartrate , pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 290K |
