1ZUU
Crystal structure of the yeast Bzz1 first SH3 domain at 0.97-A resolution
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | EMBL/DESY, HAMBURG BEAMLINE BW7A |
| Synchrotron site | EMBL/DESY, HAMBURG |
| Beamline | BW7A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-02-18 |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.976 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 36.740, 36.740, 76.550 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 20.000 - 0.970 |
| R-factor | 0.12407 |
| Rwork | 0.123 |
| R-free | 0.14220 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1e6h |
| RMSD bond length | 0.019 |
| RMSD bond angle | 1.904 |
| Data scaling software | XDS |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 1.000 |
| High resolution limit [Å] | 0.970 | 0.970 |
| Number of reflections | 31881 | |
| <I/σ(I)> | 17.4 | 3.7 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 10.2 | 7.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 295 | VAPOR DIFFUSION, HANGING DROP, temperature 295K |
