1ZUR
Crystal structure of spin labeled T4 Lysozyme (V131R1F)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X8C |
| Synchrotron site | NSLS |
| Beamline | X8C |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2002-02-11 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.9780 |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 60.059, 60.059, 96.082 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 51.990 - 1.600 |
| R-factor | 0.19068 |
| Rwork | 0.189 |
| R-free | 0.21404 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1c6t |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.542 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 100.000 | 1.660 |
| High resolution limit [Å] | 1.600 | 1.600 |
| Number of reflections | 25979 | |
| <I/σ(I)> | 17.2 | 4.6 |
| Completeness [%] | 96.0 | 96.9 |
| Redundancy | 11.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.8 | 293 | potassium phosphate, sodium phospahte, sodium choloride, sodium azide, oxidized beta-mercaptoehtanol, isopropanol, pH 6.8, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
