1ZUO
Structure of Human Ubiquitin-Conjugating Enzyme (UBCi) Involved in Embryo Attachment and Implantation
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | CHESS BEAMLINE F1 |
Synchrotron site | CHESS |
Beamline | F1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2005-05-09 |
Detector | ADSC QUANTUM 210 |
Spacegroup name | I 4 2 2 |
Unit cell lengths | 126.442, 126.442, 104.872 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 27.080 - 1.800 |
R-factor | 0.20143 |
Rwork | 0.200 |
R-free | 0.23239 |
Structure solution method | SAD |
RMSD bond length | 0.016 |
RMSD bond angle | 1.476 |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Phasing software | SOLVE |
Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 27.070 | 1.860 |
High resolution limit [Å] | 1.800 | 1.800 |
Rmerge | 0.059 | |
Number of reflections | 36041 | |
<I/σ(I)> | 22.5 | 2.4 |
Completeness [%] | 91.4 | 94.2 |
Redundancy | 2.8 | 2.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 5.8 | 298 | NACL, SODIUM CACODYLATE, VAPOR DIFFUSION, HANGING DROP, temperature 298.0K, pH 5.8 |