1ZU4
Crystal structure of FtsY from Mycoplasma mycoides- space group P21212
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | EMBL/DESY, HAMBURG BEAMLINE X11 |
| Synchrotron site | EMBL/DESY, HAMBURG |
| Beamline | X11 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 1999-09-19 |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.91 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 68.735, 101.129, 42.533 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 20.000 - 1.950 |
| R-factor | 0.21302 |
| Rwork | 0.207 |
| R-free | 0.26429 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1fts |
| RMSD bond length | 0.017 |
| RMSD bond angle | 1.624 |
| Data reduction software | DENZO |
| Data scaling software | CCP4 ((SCALA)) |
| Phasing software | CNS |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 56.800 | 1.970 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.069 | 0.321 |
| Number of reflections | 22061 | |
| Completeness [%] | 95.7 | 79.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.6 | 291 | Ammonium sulfate, potassium tartrate, Magnesium Chloride, sodium citrate, pH 5.6, VAPOR DIFFUSION, HANGING DROP, temperature 291K |
