1ZU3
Crystal Structure Of Mutant K8A Of Scorpion alpha-Like Neurotoxin Bmk M1 From Buthus Martensii Karsch
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | ENRAF-NONIUS FR591 |
| Temperature [K] | 293 |
| Detector technology | CCD |
| Collection date | 2003-01-12 |
| Detector | BRUKER SMART 2000 |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 47.237, 43.069, 25.463 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 31.800 - 1.330 |
| R-factor | 0.172 |
| Rwork | 0.172 |
| R-free | 0.19000 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1SN1.pdb |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.700 |
| Data reduction software | PROTEUM PLUS (R) |
| Data scaling software | PROTEUM PLUS (R) |
| Phasing software | AMoRE |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 31.830 | 1.410 |
| High resolution limit [Å] | 1.330 | 1.330 |
| Rmerge | 0.042 | 0.116 |
| Number of reflections | 9950 | |
| <I/σ(I)> | 12.3 | 3.5 |
| Completeness [%] | 77.0 | 41.3 |
| Redundancy | 2.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 293 | 30% (w/v) PEG8000, 0.1M sodium cacodylate pH 6.5, 0.2M ammonium sulfate, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
