1ZTY

Crystal Structure of the Chitin Oligasaccharide Binding Protein

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X4A
Synchrotron site	NSLS
Beamline	X4A
Temperature [K]	100
Detector technology	CCD
Collection date	2004-03-23
Detector	ENRAF-NONIUS
Wavelength(s)	0.97966, 0.97948, 0.97176
Spacegroup name	P 32 2 1
Unit cell lengths	92.813, 92.813, 137.029
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	50.000 - 2.300
R-factor	0.1941
Rwork	0.194
R-free	0.21950
Structure solution method	MAD
RMSD bond length	0.014
RMSD bond angle	1.730
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	CNS
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.380
High resolution limit [Å]	2.300	2.300
Rmerge	0.124	0.361
Number of reflections	30939
<I/σ(I)>	26.4	10.7
Completeness [%]	100.0	100
Redundancy	42.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8	298	ammonium sulfate, PEG400, HEPES, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 298K