1ZTX
West Nile Virus Envelope Protein DIII in complex with neutralizing E16 antibody Fab
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 4.2.2 |
| Synchrotron site | ALS |
| Beamline | 4.2.2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2004-08-05 |
| Detector | BRUKER PROTEUM |
| Wavelength(s) | 1.55 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 52.400, 83.300, 110.600 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 50.000 - 2.500 |
| R-factor | 0.226 |
| Rwork | 0.208 |
| R-free | 0.28200 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | PDB ENTRIES 1S6N 2igf |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.500 |
| Data scaling software | d*TREK (9.2LDz) |
| Phasing software | MOLREP |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 47.320 | 2.590 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Rmerge | 0.083 | 0.306 |
| Number of reflections | 16985 | |
| <I/σ(I)> | 11.3 | 2.7 |
| Completeness [%] | 97.6 | 82.7 |
| Redundancy | 3.53 | 1.99 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 293 | PEG 4000, Glycine, Hepes, pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
