1ZTM

Structure of the Uncleaved Paramyxovirus (hPIV3) Fusion Protein

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 5ID-B
Synchrotron site	APS
Beamline	5ID-B
Temperature [K]	100
Detector technology	CCD
Detector	MARRESEARCH
Wavelength(s)	0.9999
Spacegroup name	P 21 21 21
Unit cell lengths	105.560, 122.170, 195.710
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	30.020 - 3.050
R-factor	0.217
Rwork	0.217
R-free	0.26400
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.008
RMSD bond angle	1.400
Data scaling software	SCALA
Phasing software	DM
Refinement software	CNS (1.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.020	3.210
High resolution limit [Å]	3.050	3.050
Number of reflections	48676
Completeness [%]		99.6
Redundancy	10.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	301	PEG 3000, Tris, NaCl, sodium citrate, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 301K
1	VAPOR DIFFUSION, HANGING DROP	6.5	301	PEG 3000, Tris, NaCl, sodium citrate, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 301K