1ZTG
human alpha polyC binding protein KH1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU200 |
| Temperature [K] | 100 |
| Detector technology | AREA DETECTOR |
| Collection date | 2004-12-21 |
| Detector | MARRESEARCH |
| Wavelength(s) | 1.54 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 45.600, 76.800, 61.400 |
| Unit cell angles | 90.00, 111.70, 90.00 |
Refinement procedure
| Resolution | 20.000 - 3.000 |
| R-factor | 0.24012 |
| Rwork | 0.240 |
| R-free | 0.29285 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1EC6 (with oligonucleotide removed) |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.317 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 45.800 | 3.110 |
| High resolution limit [Å] | 3.000 | 3.000 |
| Rmerge | 0.057 | 0.658 |
| Number of reflections | 7978 | |
| <I/σ(I)> | 22.1 | 2.2 |
| Completeness [%] | 99.8 | 99.8 |
| Redundancy | 4.7 | 5.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 310 | cacodylate, MPD, magnesium acetate, NaCl, EDTA, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 310K |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | cacodylate | ||
| 10 | 1 | 2 | EDTA | ||
| 2 | 1 | 1 | MPD | ||
| 3 | 1 | 1 | magnesium acetate | ||
| 4 | 1 | 1 | NaCl | ||
| 5 | 1 | 1 | EDTA | ||
| 6 | 1 | 2 | cacodylate | ||
| 7 | 1 | 2 | MPD | ||
| 8 | 1 | 2 | magnesium acetate | ||
| 9 | 1 | 2 | NaCl |
