1ZTD
Hypothetical Protein Pfu-631545-001 From Pyrococcus furiosus
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-ID |
| Synchrotron site | APS |
| Beamline | 22-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2004-04-01 |
| Detector | MARMOSAIC 225 mm CCD |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 36.352, 61.093, 51.582 |
| Unit cell angles | 90.00, 97.62, 90.00 |
Refinement procedure
| Resolution | 35.970 - 2.000 |
| R-factor | 0.234 |
| Rwork | 0.234 |
| R-free | 0.27200 |
| Structure solution method | SAD |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.900 |
| Data reduction software | HKL-2000 |
| Data scaling software | SAINT |
| Phasing software | SGXPRO |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 35.970 | 2.070 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.083 | 0.229 |
| Number of reflections | 15035 | |
| <I/σ(I)> | 18.72 | 5.07 |
| Completeness [%] | 98.5 | 86.3 |
| Redundancy | 9.92 | 7.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 8.2 | 291 | 5% w/v PEG-8000, pH 8.2, Sitting Drop, Vapor Diffusion, temperature 291K, pH 8.20 |
