1ZTB

Crystal Structure of Chorismate Synthase from Mycobacterium tuberculosis

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	LNLS BEAMLINE D03B-MX1
Synchrotron site	LNLS
Beamline	D03B-MX1
Temperature [K]	100
Detector technology	CCD
Collection date	2004-03-22
Detector	MARRESEARCH
Wavelength(s)	1.427
Spacegroup name	P 64 2 2
Unit cell lengths	129.756, 129.756, 156.795
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	52.930 - 2.650
R-factor	0.16803
Rwork	0.162
R-free	0.22118
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1qxo
RMSD bond length	0.021
RMSD bond angle	2.655
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	AMoRE
Refinement software	REFMAC (5.2)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	52.930	2.790
High resolution limit [Å]	2.650	2.650
Rmerge	0.070	0.337
Number of reflections	22383
Completeness [%]	97.3	95.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.8	298	PEG 400, magnesium chloride, Hepes, pH 7.8, VAPOR DIFFUSION, HANGING DROP, temperature 298K