1ZT9

E. coli trp repressor, tetragonal crystal form

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X26C
Synchrotron site	NSLS
Beamline	X26C
Temperature [K]	100
Detector technology	CCD
Collection date	2003-10-19
Detector	ADSC QUANTUM 4
Spacegroup name	P 43
Unit cell lengths	81.348, 81.348, 72.687
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	30.000 - 2.000
R-factor	0.167
Rwork	0.164
R-free	0.21900
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2WRP BIOLOGICAL UNIT
RMSD bond length	0.013
RMSD bond angle	1.203
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	AMoRE
Refinement software	REFMAC (refmac_5.1.24)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.070
High resolution limit [Å]	2.000	2.000
Rmerge	0.063	0.169
Number of reflections	31825
Completeness [%]	99.1
Redundancy	4.3	3.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1		5.6	294	2.8 M ammonium sulfate plus 50 mM sodium, potassium phosphate buffer, 5 mM L-tryptophan, VAPOR DIFFUSION, HANGING DROP, temperature 294K, pH 5.60