1ZT3

C-terminal domain of Insulin-like Growth Factor Binding Protein-1 isolated from human amniotic fluid

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU RU300
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2004-02-17
Detector	MARRESEARCH
Wavelength(s)	1.5418
Spacegroup name	P 21 21 2
Unit cell lengths	38.540, 60.390, 31.240
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	18.920 - 1.800
R-factor	0.2236
Rwork	0.221
R-free	0.27503
Structure solution method	MIR
RMSD bond length	0.008
RMSD bond angle	1.082
Data reduction software	AUTOMAR
Phasing software	SHARP
Refinement software	REFMAC (5.2.0005)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	18.920	1.900
High resolution limit [Å]	1.800	1.800
Number of reflections	7168
<I/σ(I)>	14.2	2.8
Completeness [%]	99.5	98.1
Redundancy	6.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	277	dioxane, pH 7.5, VAPOR DIFFUSION, SITTING DROP, temperature 277K