1ZT1
crystal structure of class I MHC H-2Kk in complex with an octapeptide
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-4 |
| Synchrotron site | ESRF |
| Beamline | ID14-4 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2003-02-03 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.9763 |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 111.758, 111.758, 109.417 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 14.870 - 2.500 |
| R-factor | 0.21202 |
| Rwork | 0.210 |
| R-free | 0.25141 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1leg |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.157 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.1.24) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 15.000 | 2.640 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Rmerge | 0.087 | 0.419 |
| Number of reflections | 27403 | |
| <I/σ(I)> | 8.9 | 2.1 |
| Completeness [%] | 99.0 | 99.2 |
| Redundancy | 2.7 | 2.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 293 | NaCl, Hepes, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
