1ZSY
The structure of human mitochondrial 2-enoyl thioester reductase (CGI-63)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-05-10 |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.984 |
| Spacegroup name | I 2 2 2 |
| Unit cell lengths | 74.190, 117.184, 127.372 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 39.900 - 1.750 |
| R-factor | 0.17104 |
| Rwork | 0.170 |
| R-free | 0.19500 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1gu7 |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.419 |
| Data reduction software | MOSFLM |
| Data scaling software | CCP4 ((SCALA)) |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 44.600 | 1.840 |
| High resolution limit [Å] | 1.750 | 1.750 |
| Rmerge | 0.128 | 0.492 |
| Number of reflections | 55668 | |
| <I/σ(I)> | 12.2 | 3 |
| Completeness [%] | 99.1 | 100 |
| Redundancy | 4.6 | 4.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 293 | 1.6M Ammonium Sulphate, 0.16M SODIUM CHLORIDE, 0.08M Sodium Cacodylate , pH 6.5, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
