1ZSX
Crystal Structure Of Human Potassium Channel Kv Beta-subunit (KCNAB2)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-04-23 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 0.97910 |
| Spacegroup name | I 4 2 2 |
| Unit cell lengths | 113.231, 113.231, 142.505 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 44.300 - 1.900 |
| R-factor | 0.14623 |
| Rwork | 0.145 |
| R-free | 0.17240 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1QRQ.pdb |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.522 |
| Data reduction software | MOSFLM |
| Data scaling software | CCP4 ((SCALA)) |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 44.300 | 2.000 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Number of reflections | 36582 | |
| Completeness [%] | 99.7 | 99 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.2 | 293 | PEG300, sodium/potassium phosphate, sodium chloride, pH 6.2, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
