1ZSW
Crystal Structure of Bacillus cereus Metallo Protein from Glyoxalase family
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 32-ID |
| Synchrotron site | APS |
| Beamline | 32-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-04-10 |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.99997 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 72.509, 61.152, 96.423 |
| Unit cell angles | 90.00, 110.91, 90.00 |
Refinement procedure
| Resolution | 25.000 - 1.650 |
| R-factor | 0.17769 |
| Rwork | 0.175 |
| R-free | 0.22238 |
| Structure solution method | SAD |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.475 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | SOLVE |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 25.000 | 1.710 |
| High resolution limit [Å] | 1.650 | 1.650 |
| Rmerge | 0.071 | 0.343 |
| Number of reflections | 47459 | |
| <I/σ(I)> | 21.8 | 5.9 |
| Completeness [%] | 99.7 | 99.1 |
| Redundancy | 7.4 | 6.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 295 | 0.1 M MES, 25% w/v PEG 8000, pH 6.5, VAPOR DIFFUSION, SITTING DROP, temperature 295K |
