1ZSO

Hypothetical protein from plasmodium falciparum

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 8.2.2
Synchrotron site	ALS
Beamline	8.2.2
Temperature [K]	100
Detector technology	CCD
Collection date	2004-10-13
Detector	ADSC QUANTUM 315
Wavelength(s)	0.9795, 0.9797, 0.9537
Spacegroup name	P 21 21 21
Unit cell lengths	62.247, 71.104, 78.616
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	20.000 - 2.170
R-factor	0.18585
Rwork	0.183
R-free	0.23262
Structure solution method	MAD
RMSD bond length	0.017
RMSD bond angle	1.584
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	SOLVE (2.08)
Refinement software	REFMAC (5.2.0005)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	2.230
High resolution limit [Å]	2.150	4.630	2.150
Rmerge		0.049	0.365
Number of reflections	18151
<I/σ(I)>		45.512	1.636
Completeness [%]	92.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8	298	0.4 ul protein 12.4 mg/ml, 0.4 ul crystallization buffer, 1.0M LiCl, 25% PEG 6000, 0.025M Mg nitrate, 0.1M Tris, pH 8.0, VAPOR DIFFUSION, SITTING DROP, temperature 298K