1ZSE
RNA stemloop from bacteriophage Qbeta complexed with an N87S mutant MS2 Capsid
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SRS BEAMLINE PX14.1 |
| Synchrotron site | SRS |
| Beamline | PX14.1 |
| Temperature [K] | 298 |
| Detector technology | CCD |
| Collection date | 2001-11-26 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 1.488 |
| Spacegroup name | H 3 2 |
| Unit cell lengths | 287.090, 287.090, 651.150 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 30.000 - 3.000 |
| R-factor | 0.225 |
| Rwork | 0.225 |
| R-free | 0.23200 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2ms2 |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.500 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 3.220 |
| High resolution limit [Å] | 3.000 | 3.020 |
| Number of reflections | 141000 | |
| <I/σ(I)> | 3.1 | 1.5 |
| Completeness [%] | 70.3 | 70.9 |
| Redundancy | 2.6 | 1.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.4 | 303 | MS2 COATPROTEIN IN 1.25% OR 1.5% PEG 8K, 0.4M SODIUM PHOSPHATE BUFFER, pH 7.4, VAPOR DIFFUSION, HANGING DROP, temperature 303K |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | MS2 | ||
| 2 | 1 | 1 | PEG | ||
| 3 | 1 | 1 | SODIUM PHOSPHATE | ||
| 4 | 1 | 2 | MS2 | ||
| 5 | 1 | 2 | SODIUM PHOSPHATE |
