1ZSD

Crystal Structure Of HLA-B*3501 Presenting an 11-Mer EBV Antigen EPLPQGQLTAY

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 14-BM-C
Synchrotron site	APS
Beamline	14-BM-C
Detector technology	CCD
Collection date	2003-11-25
Detector	ADSC QUANTUM 4
Wavelength(s)	1.5418
Spacegroup name	P 21 21 21
Unit cell lengths	50.930, 81.490, 109.920
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	25.500 - 1.700
R-factor	0.219
Rwork	0.219
R-free	0.24600
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1a1n
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	CNS
Refinement software	CNS

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	100.000	100.000	1.760
High resolution limit [Å]	1.700	3.660	1.700
Rmerge	0.079	0.041	0.667
Number of reflections	47652
Completeness [%]	93.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.6		0.2M ammonium acetate, 17% w/v PEG 3350, 100mM cacodylate, pH 7.6, VAPOR DIFFUSION, HANGING DROP