1ZSB

CARBONIC ANHYDRASE II MUTANT E117Q, TRANSITION STATE ANALOGUE ACETAZOLAMIDE

Experimental procedure

Detector technology	IMAGE PLATE
Collection date	1994-08-12
Detector	RIGAKU RAXIS IIC
Spacegroup name	P 1 21 1
Unit cell lengths	42.700, 41.700, 73.000
Unit cell angles	90.00, 104.60, 90.00

Refinement procedure

Resolution	8.000 - 2.000
R-factor	0.164
Rwork	0.164
RMSD bond length	0.013
RMSD bond angle	25.700 *
Data reduction software	MOSFLM
Phasing software	X-PLOR
Refinement software	X-PLOR

Data quality characteristics

	Overall
Low resolution limit [Å]	20.200
High resolution limit [Å]	2.000
Rmerge	0.030
Total number of observations	68852 *
Number of reflections	15793
Completeness [%]	93.0
Redundancy	4.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, sitting drop *	8 *	25 *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	reservoir	Tris-HCl	50 (mM)
2	1	reservoir		50-75 (%sat)
3	1	drop	protain	0.3 (mM)
4	1	drop	Tris-HCl	50 (mM)