1ZSA

CARBONIC ANHYDRASE II MUTANT E117Q, APO FORM

Experimental procedure

Detector technology	IMAGE PLATE
Collection date	1995-07-16
Detector	RIGAKU RAXIS IIC
Spacegroup name	P 1 21 1
Unit cell lengths	42.700, 41.700, 73.000
Unit cell angles	90.00, 104.60, 90.00

Refinement procedure

Resolution	8.000 - 2.500
R-factor	0.168
Rwork	0.168
RMSD bond length	0.013
RMSD bond angle	25.900 *
Data reduction software	MOSFLM
Phasing software	X-PLOR
Refinement software	X-PLOR

Data quality characteristics

	Overall
Low resolution limit [Å]	10.000
High resolution limit [Å]	2.500
Rmerge	0.080
Total number of observations	24469 *
Number of reflections	7871
Completeness [%]	90.0
Redundancy	3.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, sitting drop *	8 *	25 *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	reservoir	Tris-HCl	50 (mM)
2	1	reservoir		50-75 (%sat)
3	1	drop	protain	0.3 (mM)
4	1	drop	Tris-HCl	50 (mM)