1ZS7
The structure of gene product APE0525 from Aeropyrum pernix
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-BM |
| Synchrotron site | APS |
| Beamline | 19-BM |
| Temperature [K] | 105 |
| Detector technology | CCD |
| Collection date | 2004-12-14 |
| Detector | CUSTOM-MADE |
| Wavelength(s) | 0.97940, 0.97959 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 48.850, 112.792, 39.001 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 36.860 - 1.850 |
| R-factor | 0.1683 |
| Rwork | 0.167 |
| R-free | 0.19883 |
| Structure solution method | MAD |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.287 |
| Data scaling software | HKL-2000 |
| Phasing software | HKL-3000 |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 36.900 | 1.920 |
| High resolution limit [Å] | 1.850 | 1.850 |
| Rmerge | 0.079 | |
| Number of reflections | 19171 | |
| <I/σ(I)> | 17.2 | 2.1 |
| Completeness [%] | 94.4 | 79.9 |
| Redundancy | 4.5 | 2.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.9 | 293 | K/Na Phosphate, glycerol, ethylene glycol, sucrose, pH 6.9, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
