1ZS6
structure of human nucleoside-diphosphate kinase 3
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU FR-E |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2005-05-16 |
| Detector | RIGAKU RAXIS IV |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 86.614, 86.614, 126.856 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 50.000 - 2.300 |
| R-factor | 0.21297 |
| Rwork | 0.211 |
| R-free | 0.25536 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1be4 |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.284 |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.380 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Rmerge | 0.119 | |
| Number of reflections | 21910 | |
| Completeness [%] | 99.0 | 98 |
| Redundancy | 14.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 291 | Sodium Citrate, pH 7.5, VAPOR DIFFUSION, SITTING DROP, temperature 291K |
