1ZS4
Structure of bacteriophage lambda cII protein in complex with DNA
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X9A |
| Synchrotron site | NSLS |
| Beamline | X9A |
| Detector technology | CCD |
| Collection date | 2004-08-24 |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.98396, 0.97903, 0.97922, 0.96384 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 44.771, 97.132, 59.869 |
| Unit cell angles | 90.00, 105.36, 90.00 |
Refinement procedure
| Resolution | 50.000 - 1.700 |
| Rwork | 0.209 |
| R-free | 0.22800 |
| Structure solution method | MAD |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | MLPHARE |
| Refinement software | CNS (1.0) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 50.000 |
| High resolution limit [Å] | 1.700 |
| Number of reflections | 54170 |
| <I/σ(I)> | 8.7 |
| Completeness [%] | 97.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 4.8 | 295 | PEG 550 MME, SODIUM ACETATE, pH 4.8, VAPOR DIFFUSION, temperature 295K |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | PEG 550 | ||
| 2 | 1 | 1 | Sodium acetate | ||
| 3 | 1 | 2 | PEG 550 |
