1ZRZ
Crystal Structure of the Catalytic Domain of Atypical Protein Kinase C-iota
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2004-08-09 |
| Detector | MAR CCD 165 mm |
| Wavelength(s) | 1.0003 |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 78.143, 78.143, 112.625 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 24.940 - 3.000 |
| R-factor | 0.249 |
| Rwork | 0.249 |
| R-free | 0.33300 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | protein kinase C-thate PDB ENTRY 1XJD |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.500 |
| Data reduction software | MOSFLM |
| Data scaling software | CCP4 ((SCALA)) |
| Phasing software | MOLREP |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 37.000 | 3.190 |
| High resolution limit [Å] | 3.000 | 3.000 |
| Rmerge | 0.093 | 0.371 |
| Number of reflections | 7778 | |
| <I/σ(I)> | 6.5 | 2 |
| Completeness [%] | 92.9 | 100 |
| Redundancy | 5.7 | 6.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 293 | PEG 400, sodium acetate, MES, pH 6.5, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
