1ZRS
wild-type LD-carboxypeptidase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | MPG/DESY, HAMBURG BEAMLINE BW6 |
| Synchrotron site | MPG/DESY, HAMBURG |
| Beamline | BW6 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2004-11-21 |
| Detector | MARRESEARCH |
| Wavelength(s) | 1.05 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 52.185, 78.271, 71.258 |
| Unit cell angles | 90.00, 104.33, 90.00 |
Refinement procedure
| Resolution | 20.000 - 1.500 |
| R-factor | 0.16442 |
| Rwork | 0.164 |
| R-free | 0.17730 |
| Structure solution method | MIRAS |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.460 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | MLPHARE |
| Refinement software | REFMAC (5.1.24) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 1.520 |
| High resolution limit [Å] | 1.500 | 1.500 |
| Rmerge | 0.037 | 0.252 |
| Number of reflections | 86224 | |
| <I/σ(I)> | 20.9 | 4 |
| Completeness [%] | 97.1 | 95.1 |
| Redundancy | 2.2 | 2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 4.5 | 294 | citric acid, pH 4.5, VAPOR DIFFUSION, SITTING DROP, temperature 294K |
