1ZR8
Crystal Structure of the complex formed between group II phospholipase A2 and a plant alkaloid ajmaline at 2.0A resolution
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU300 |
| Temperature [K] | 300 |
| Detector technology | IMAGE PLATE |
| Collection date | 2005-04-12 |
| Detector | MARRESEARCH |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 43 |
| Unit cell lengths | 53.257, 53.257, 48.657 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 20.000 - 2.030 |
| R-factor | 0.1896 |
| Rwork | 0.186 |
| R-free | 0.22146 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1skg |
| RMSD bond length | 0.014 |
| RMSD bond angle | 3.925 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 54.500 | 2.120 |
| High resolution limit [Å] | 2.030 | 2.030 |
| Number of reflections | 8322 | |
| <I/σ(I)> | 10.9 | 2.8 |
| Completeness [%] | 98.0 | 94.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 298 | 0.2M AMMONIUM SULPHATE, 30% PEG, pH 7.0, VAPOR DIFFUSION, SITTING DROP, temperature 298K |
