1ZR6

The crystal structure of an Acremonium strictum glucooligosaccharide oxidase reveals a novel flavinylation

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE AR-NW12A
Synchrotron site	Photon Factory
Beamline	AR-NW12A
Temperature [K]	100
Detector technology	CCD
Collection date	2004-11-24
Detector	ADSC QUANTUM 4
Wavelength(s)	0.7
Spacegroup name	P 21 21 21
Unit cell lengths	53.084, 90.907, 111.679
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	50.000 - 1.550
R-factor	0.18
Rwork	0.180
R-free	0.20000
Structure solution method	SAD
RMSD bond length	0.005
RMSD bond angle	1.390
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	OASIS
Refinement software	CNS (1.0)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.580
High resolution limit [Å]	1.550	1.550
Rmerge	0.061	0.270
Number of reflections	77613
<I/σ(I)>	30	3.3
Completeness [%]	98.1	95.9
Redundancy	11	6.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	295	25% PEGMME 550, 10mM ZnSO4, 0.1M MES, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 295K
1	VAPOR DIFFUSION, HANGING DROP	6.5	295	25% PEGMME 550, 10mM ZnSO4, 0.1M MES, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 295K