1ZR2
Structure of a Synaptic gamma-delta Resolvase Tetramer Covalently Linked to two Cleaved DNAs
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | CHESS BEAMLINE A1 |
| Synchrotron site | CHESS |
| Beamline | A1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2003-02-14 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.909 |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 125.050, 125.050, 127.390 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 34.500 - 3.900 |
| R-factor | 0.26975 |
| Rwork | 0.264 |
| R-free | 0.32237 |
| Structure solution method | MIR |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.329 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | SOLVE |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 41.000 | |
| High resolution limit [Å] | 3.900 | 3.900 |
| Number of reflections | 10987 | |
| <I/σ(I)> | 11.9 | 11.9 |
| Completeness [%] | 94.9 | 94.9 |
| Redundancy | 3 | 3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4 | 298 | 0.2M Sodium Chloride, 0.1M Magnesium Formate, 0.1M Sodium Acetate, pH 4, VAPOR DIFFUSION, HANGING DROP, temperature 25K |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | Sodium Chloride | ||
| 2 | 1 | 1 | Magnesium Formate | ||
| 3 | 1 | 1 | Sodium Acetate | ||
| 4 | 1 | 1 | H2O | ||
| 5 | 1 | 2 | Sodium Chloride | ||
| 6 | 1 | 2 | Magnesium Formate | ||
| 7 | 1 | 2 | Sodium Acetate | ||
| 8 | 1 | 2 | H2O |
