1ZPT
Escherichia coli Methylenetetrahydrofolate Reductase (reduced) complexed with NADH, pH 7.25
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2003-09-23 |
| Detector | RIGAKU |
| Wavelength(s) | 1.5418 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 103.613, 127.735, 97.831 |
| Unit cell angles | 90.00, 121.73, 90.00 |
Refinement procedure
| Resolution | 19.900 - 1.950 |
| R-factor | 0.219 |
| Rwork | 0.219 |
| R-free | 0.24600 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1b5t |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.400 |
| Data scaling software | SCALEPACK |
| Phasing software | EPMR |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 19.900 | 2.020 |
| High resolution limit [Å] | 1.950 | 1.950 |
| Number of reflections | 76927 | |
| <I/σ(I)> | 19.4 | 3.75 |
| Completeness [%] | 97.9 | 96.4 |
| Redundancy | 3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.25 | 295 | PEG 4000, LITHIUM SULFATE, SODIUM CACODYLATE, ETHANOL, MESO-ERYTHRITOL, pH 7.25, VAPOR DIFFUSION, HANGING DROP, temperature 295.0K |
