1ZPS
Crystal structure of Methanobacterium thermoautotrophicum phosphoribosyl-AMP cyclohydrolase HisI
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X8C |
Synchrotron site | NSLS |
Beamline | X8C |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2001-10-26 |
Detector | ADSC QUANTUM 4 |
Wavelength(s) | 1.0091 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 39.783, 54.358, 117.362 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 39.900 - 1.700 |
R-factor | 0.208 |
Rwork | 0.207 |
R-free | 0.23500 |
Structure solution method | SAD |
RMSD bond length | 0.004 |
RMSD bond angle | 1.190 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | SOLVE |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 39.900 | 1.760 |
High resolution limit [Å] | 1.700 | 1.700 |
Number of reflections | 47949 | |
<I/σ(I)> | 28.9 | 5.5 |
Completeness [%] | 91.3 | 70.3 |
Redundancy | 3.3 | 1.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 293 | 50 mM CdSO4, 1.6 M Na Acetate, 10% (w/v) glycerol, 100 mM HEPES, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |