1ZPS
Crystal structure of Methanobacterium thermoautotrophicum phosphoribosyl-AMP cyclohydrolase HisI
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X8C |
| Synchrotron site | NSLS |
| Beamline | X8C |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2001-10-26 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 1.0091 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 39.783, 54.358, 117.362 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 39.900 - 1.700 |
| R-factor | 0.208 |
| Rwork | 0.207 |
| R-free | 0.23500 |
| Structure solution method | SAD |
| RMSD bond length | 0.004 |
| RMSD bond angle | 1.190 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SOLVE |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 39.900 | 1.760 |
| High resolution limit [Å] | 1.700 | 1.700 |
| Number of reflections | 47949 | |
| <I/σ(I)> | 28.9 | 5.5 |
| Completeness [%] | 91.3 | 70.3 |
| Redundancy | 3.3 | 1.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 293 | 50 mM CdSO4, 1.6 M Na Acetate, 10% (w/v) glycerol, 100 mM HEPES, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
