1ZPL

E. coli F17a-G lectin domain complex with GlcNAc(beta1-O)Me

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Temperature [K]	100
Detector technology	CCD
Collection date	2004-06-01
Detector	ADSC QUANTUM 4
Spacegroup name	P 31 2 1
Unit cell lengths	42.805, 42.805, 288.244
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	20.000 - 1.700
R-factor	0.2123
Rwork	0.208
R-free	0.25440
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	PDB 1O9V CHAIN A
RMSD bond length	0.007
RMSD bond angle	1.500
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	AMoRE
Refinement software	CNS (1.0)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.760
High resolution limit [Å]	1.700	1.700
Rmerge		0.335
Number of reflections	35422
Completeness [%]		70
Redundancy		1.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	293	10% (v/v) isopropanol, 20% (w/v) PEG 4000, 100 mM Hepes, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K