1ZPG

Arginase I covalently modified with propylamine at Q19C

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	CHESS BEAMLINE F1
Synchrotron site	CHESS
Beamline	F1
Temperature [K]	100
Detector technology	CCD
Collection date	2002-09-01
Detector	ADSC QUANTUM 4
Wavelength(s)	.9124
Spacegroup name	P 32
Unit cell lengths	88.184, 88.184, 106.652
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	28.870 - 1.900
R-factor	0.198
Rwork	0.198
R-free	0.22000
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.006
RMSD bond angle	1.300
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	AMoRE
Refinement software	CNS (1.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	28.870	2.000
High resolution limit [Å]	1.900	1.900
Rmerge	0.057	0.164
Number of reflections	72508
Completeness [%]		99.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8.4	277	Bicine, PEG 8000, MnCl2, pH 8.4, VAPOR DIFFUSION, HANGING DROP, temperature 277K