1ZP9
Crystal Structure of full-legnth A.fulgidus Rio1 Serine Kinase bound to ATP and Mn2+ ions.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-ID |
| Synchrotron site | APS |
| Beamline | 22-ID |
| Detector technology | IMAGE PLATE |
| Collection date | 2004-11-05 |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.96860 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 53.306, 80.373, 121.320 |
| Unit cell angles | 90.00, 90.02, 90.00 |
Refinement procedure
| Resolution | 30.000 - 2.000 |
| R-factor | 0.18067 |
| Rwork | 0.177 |
| R-free | 0.24880 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1ztf |
| RMSD bond length | 0.018 |
| RMSD bond angle | 1.679 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 30.000 |
| High resolution limit [Å] | 2.000 |
| Number of reflections | 63199 |
| <I/σ(I)> | 11.2 |
| Redundancy | 3.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 6.3 | 293 | PEG 4000, Ammonium Sulfate, MES, pH 6.3, VAPOR DIFFUSION, temperature 293K |
