1ZP8
HIV Protease with inhibitor AB-2
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-ID |
| Synchrotron site | APS |
| Beamline | 22-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2003-08-07 |
| Detector | MAR CCD 165 mm |
| Wavelength(s) | 1.0089 |
| Spacegroup name | P 61 2 2 |
| Unit cell lengths | 63.100, 63.100, 82.100 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 10.000 - 2.020 |
| R-factor | 0.197 |
| Rwork | 0.197 |
| R-free | 0.32600 |
| Structure solution method | REFINEMENT |
| Starting model (for MR) | 3tlh |
| RMSD bond length | 0.012 |
| RMSD bond angle | 0.042 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | SHELX |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.090 |
| High resolution limit [Å] | 2.020 | 2.020 |
| Number of reflections | 5931 | |
| <I/σ(I)> | 34.4 | 2.94 |
| Completeness [%] | 95.9 | 67 |
| Redundancy | 10.8 | 2.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5 | 293 | 1.34 M Ammonium Sulfate, 0.1 M Na Acetate, pH 4.8-5.4, pH 5.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
